Commission Implementing Regulation (EU) 2020/1201 of 14 August 2020 as regards measures to prevent the introduction into and the spread within the Union of Xylella fastidiosa (Wells et al.)

• 1. In the region of Οccitania:
• (a) Municipalities located in the department of Ariège: Aigues-Juntes, Allières, Alzen, Arabaux, Artigat, Artix, Arvigna, La Bastide-de-Besplas, La Bastide-de-Bousignac, La Bastide-de-Lordat, La Bastide-de-Sérou, Baulou, Benagues, Besset, Bonnac, Bordes-sur-Arize, Brassac, Brie, Bédeille, Bénac, Bézac, Cadarcet, Calzan, Camarade, Camon, Campagne-sur-Arize, Canté, Carla-Bayle, Carla-de-Roquefort, Carlaret, Castex, Castéras, Cazals-des-Baylès, Cazaux, Clermont, Contrazy, Cos, Coussa, Coutens, Crampagna, Cérizols, Dalou, Daumazan-sur-Arize, Dun, Durban-sur-Arize, Durfort, Escosse, Esplas, Fabas, Ferrières-sur-Ariège, Foix, Fornex, Fossat, Gabre, Ganac, Gaudiès, Gudas, Herm, Issards, Justiniac, Labatut, Lagarde, Lanoux, Lapenne, Lasserre, Lescousse, Lescure, Leychert, Lieurac, Limbrassac, Lissac, Loubaut, Loubens, Loubières, Ludiès, Lézat-sur-Lèze, Madière, Malegoude, Malléon, Manses, Le Mas-d’Azil, Mauvezin-de-Sainte-Croix, Mazères, Mirepoix, Monesple, Montardit, Montaut, Montels, Montesquieu-Avantès, Montfa, Montgailhard, Montjoie-en-Couserans, Montseron, Montégut-Plantaurel, Moulin-Neuf, Méras, Mérigon, Pailhès, Pamiers, Pradettes, Pradières, Pujols, Rieucros, Rieux-de-Pelleport, Rimont, Roquefort-les-Cascades, Roumengoux, Sabarat, Saint-Amadou, Saint-Bauzeil, Saint-Félix-de-Rieutord, Saint-Félix-de-Tournegat, Saint-Jean-de-Verges, Saint-Jean-du-Falga, Saint-Julien-de-Gras-Capou, Saint-Martin-d’Oydes, Saint-Martin-de-Caralp, Saint-Michel, Saint-Pierre-de-Rivière, Saint-Quentin-la-Tour, Saint-Quirc, Saint-Victor-Rouzaud, Saint-Ybars, Sainte-Croix-Volvestre, Sainte-Foi, Sainte-Suzanne, Saverdun, Serres-sur-Arget, Sieuras, Soula, Suzan, Ségura, Teilhet, Thouars-sur-Arize, Tour-du-Crieu, Tourtouse, Tourtrol, Troye-d'Ariège, Trémoulet, Unzent, Vals, Varilhes, Ventenac, Vernajoul, Vernet, Verniolle, Villeneuve-du-Latou, Villeneuve-du-Paréage, Vira, Viviès
• (b) Municipalities located in the department of Aude: Aigues-Vives, Airoux, Ajac, Alaigne, Alairac, Albas, Alet-les-Bains, Alzonne, Antugnac, Aragon, Argeliers, Argens-Minervois, Armissan, Arquettes-en-Val, Arzens, Azille, Badens, Bages, Bagnoles, Baraigne, Barbaira, Belcastel-et-Buc, Belflou, Bellegarde-du-Razès, Belpech, Belvèze-du-Razès, Berriac, Bezole, Bizanet, Bize-Minervois, Blomac, Bouilhonnac, Bouisse, Bourigeole, Bouriège, Boutenac, Bram, Brousses-et-Villaret, Brugairolles, Brunels, Brézilhac, Cabrespine, Cahuzac, Cailhau, Cailhavel, Cambieure, Camplong-d’Aude, Canet, Capendu, Carcassonne, Carlipa, Cascastel-des-Corbières, Cassaigne, Cassés, Castans, Castelnau-d’Aude, Castelnaudary, Castelreng, Caudebronde, Caunes-Minervois, Caunette-sur-Lauquet, Caunettes-en-Val, Caux-et-Sauzens, Cavanac, Cazalrenoux, Cazilhac, Cenne-Monestiés, Citou, Clermont-sur-Lauquet, Comigne, Conilhac-Corbières, Conques-sur-Orbiel, Corbières, Couffoulens, Cournanel, Coursan, Courtauly, Courtète, Coustouge, Cruscades, Cumiès, Cuxac-Cabardès, Cuxac-d’Aude, Cépie, Digne-d’Amont, Digne-d’Aval, Donazac, Douzens, Durban-Corbières, Embres-et-Castelmaure, Escales, Escueillens-et-Saint-Just-de-Bélengard, Fabrezan, Fajac-en-Val, Fajac-la-Relenque, Fanjeaux, Fendeille, Fenouillet-du-Razès, Ferrals-les-Corbières, Ferran, Festes-et-Saint-André, Fleury, Floure, Fontcouverte, Fonters-du-Razès, Fontiers-Cabardès, Fontiès-d'Aude, Fontjoncouse, Force, Fournes-Cabardès, Fraisse-Cabardès, Gaja-et-Villedieu, Gaja-la-Selve, Gardie, Generville, Ginestas, Gourvieille, Gramazie, Greffeil, Gruissan, Homps, Hounoux, Ilhes, Issel, Jonquières, Labastide-Esparbairenque, Labastide-d’Anjou, Labastide-en-Val, Labécède-Lauragais, Lacombe, Ladern-sur-Lauquet, Lafage, Lagrasse, Lairière, Laprade, Lasbordes, Lasserre-de-Prouille, Lastours, Laurabuc, Laurac, Lauraguel, Laure-Minervois, Lavalette, Lespinassière, Leuc, Lignairolles, Limousis, Limoux, Loupia, Louvière-Lauragais, Luc-sur-Orbieu, Lézignan-Corbières, Magrie, Mailhac, Malras, Malves-en-Minervois, Malviès, Marcorignan, Marquein, Marseillette, Martys, Mas-Cabardès, Mas-Saintes-Puelles, Mas-des-Cours, Mayreville, Mayronnes, Mazerolles-du-Razès, Miraval-Cabardès, Mirepeisset, Mireval-Lauragais, Missègre, Molandier, Molleville, Montauriol, Montbrun-des-Corbières, Montclar, Montferrand, Montgradail, Monthaut, Montirat, Montjoi, Montmaur, Montolieu, Montredon-des-Corbières, Montréal, Montséret, Monze, Moussan, Moussoulens, Moux, Mézerville, Narbonne, Névian, Ornaisons, Orsans, Ouveillan, Palaja, Paraza, Pauligne, Payra-sur-l’Hers, Pech-Luna, Pennautier, Pexiora, Peyrefitte-du-Razès, Peyrefitte-sur-l’Hers, Peyrens, Peyriac-Minervois, Peyriac-de-Mer, Pezens, Pieusse, Plaigne, Plavilla, Pomarède, Pomas, Pomy, Port-la-Nouvelle, Portel-des-Corbières, Pouzols-Minervois, Pradelles-Cabardès, Preixan, Puginier, Puichéric, Pécharic-et-le-Py, Pépieux, Raissac-d’Aude, Raissac-sur-Lampy, Redorte, Ribaute, Ribouisse, Ricaud, Rieux-Minervois, Rieux-en-Val, Roquecourbe-Minervois, Roquefort-des-Corbières, Roquefère, Roquetaillade-et-Conilhac, Roubia, Rouffiac-d’Aude, Roullens, Routier, Rustiques, Saint-Amans, Saint-André-de-Roquelongue, Saint-Benoît, Saint-Couat-d’Aude, Saint-Couat-du-Razès, Saint-Denis, Saint-Frichoux, Saint-Gaudéric, Saint-Hilaire, Saint-Jean-de-Barrou, Saint-Julien-de-Briola, Saint-Laurent-de-la-Cabrerisse, Saint-Marcel-sur-Aude, Saint-Martin-Lalande, Saint-Martin-de-Villereglan, Saint-Martin-des-Puits, Saint-Martin-le-Vieil, Saint-Michel-de-Lanès, Saint-Nazaire-d’Aude, Saint-Papoul, Saint-Paulet, Saint-Pierre-des-Champs, Saint-Polycarpe, Saint-Sernin, Sainte-Camelle, Sainte-Eulalie, Sainte-Valière, Saissac, Salles-d’Aude, Salles-sur-l’Hers, Sallèles-Cabardès, Sallèles-d’Aude, Salsigne, Salza, Seignalens, Serpent, Serviès-en-Val, Sigean, Sonnac-sur-l’Hers, Souilhanels, Souilhe, Soupex, Talairan, Taurize, Termes, Thézan-des-Corbières, Tourette-Cabardès, Tournissan, Tourouzelle, Tourreilles, Trassanel, Trausse, Trèbes, Tréville, Tréziers, Val de Lambronne, Val-de-Dagne, Ventenac-Cabardès, Ventenac-en-Minervois, Verdun-en-Lauragais, Verzeille, Vignevieille, Villalier, Villanière, Villar-Saint-Anselme, Villar-en-Val, Villardebelle, Villardonnel, Villarzel-Cabardès, Villarzel-du-Razès, Villasavary, Villautou, Villebazy, Villedaigne, Villedubert, Villefloure, Villegailhenc, Villegly, Villelongue-d’Aude, Villemagne, Villemoustaussou, Villeneuve-Minervois, Villeneuve-la-Comptal, Villeneuve-les-Corbières, Villeneuve-lès-Montréal, Villepinte, Villerouge-Termenès, Villesiscle, Villespy, Villesèque-des-Corbières, Villesèquelande, Villetritouls, Vinassan, Véraza
• (c) Municipalities located in the department of Haute-Garonne: Aignes, Auribail, Auterive, Avignonet-Lauragais, Bax, Beauteville, Bois-de-la-Pierre, Bélesta-en-Lauragais, Caignac, Calmont, Canens, Capens, Carbonne, Castagnac, Caujac, Cazères, Cintegabelle, Couladère, Esperce, Folcarde, Fousseret, Gaillac-Toulza, Gardouch, Gensac-sur-Garonne, Gibel, Goutevernisse, Gouzens, Gratens, Grazac, Labastide-Clermont, Lacaugne, Lafitte-Vigordane, Lagarde, Lagrâce-Dieu, Lahitère, Lapeyrère, Latour, Latrape, Lavelanet-de-Comminges, Longages, Mailholas, Marignac-Lasclares, Marliac, Marquefave, Massabrac, Mauressac, Mauvaisin, Monestrol, Montaut, Montberaud, Montbrun-Bocage, Montclar-Lauragais, Montesquieu-Volvestre, Montgazin, Montgeard, Mourvilles-Hautes, Nailloux, Noé, Palaminy, Peyssies, Plagne, Plan, Pouy-de-Touges, Puydaniel, Renneville, Revel, Rieumajou, Rieux-Volvestre, Roumens, Saint-Christaud, Saint-Félix-Lauragais, Saint-Julien-sur-Garonne, Saint-Michel, Saint-Sulpice-sur-Lèze, Saint-Élix-le-Château, Salles-sur-Garonne, Seyre, Vaudreuille
• (d) Municipalities located in the department of Hérault: Agel, Aigne, Aigues-Vives, Azillanet, Beaufort, Béziers, Capestang, Cassagnoles, Cers, Cesseras, Colombiers, Cruzy, Ferrals-les-Montagnes, Félines-Minervois, Lespignan, Livinière, Montady, Montels, Montouliers, Nissan-lez-Enserune, Olonzac, Oupia, Poilhes, Quarante, Sauvian, Siran, Vendres, Villeneuve-lès-Béziers, Villespassans
• (e) Municipalities located in the department of Tarn: Aiguefonde, Albine, Arfons, Aussillon, Bout-du-Pont-de-Larn, Cammazes, Dourgne, Durfort, Escoussens, Labruguière, Lacabarède, Massaguel, Mazamet, Pont-de-Larn, Saint-Amancet, Saint-Amans-Soult, Sauveterre, Sorèze, Verdalle

PART C

Infected zone in Spain

The infected zone in Spain includes the following area:

The Autonomous Community of Balearic Islands

PART D

Infected zone in Portugal

The infected zone in Portugal includes the following area:

Region of Porto

Part of the following parishes located in the municipality of Espinho:

Anta e Guetim

Espinho

Silvalde

Parishes located in the municipality of Gondomar:

Gondomar (São Cosme), Valbom e Jovim

Part of the following parishes located in the municipality of Gondomar:

Baguim do Monte (Rio Tinto)

Fânzeres e São Pedro da Cova

Foz do Sousa e Covelo

Melres e Medas

Rio Tinto

Part of the following parishes located in the municipality of Maia:

Pedrouços

Part of the following parishes located in the municipality of Matosinhos:

Custóias, Leça do Balio e Guifões

São Mamede de Infesta e Senhora da Hora

Parishes located in the municipality of Porto:

Bonfim

Campanhã

Cedofeita, Ildefonso, Sé, Miragaia, Nicolau, Vitória

Paranhos

Part of the following parishes located in the municipality of Porto:

Aldoar, Foz do Douro e Nevogilde

Lordelo do Ouro e Massarelos

Ramalde

Parishes located in the municipality of Santa Maria da Feira:

Argoncilhe

Fiães

Nogueira da Regedoura

Sanguedo

Part of the following parishes located in the municipality of Santa Maria da Feira:

Caldas de São Jorge e Pigeiros

Canedo, Vale e Vila Maior

Lobão, Gião, Louredo e Guisande

Lourosa

Mozelos

Santa Maria de Lamas

São João de Ver

São Paio de Oleiros

Municipality of Vila Nova de Gaia:

All parishes

ANNEX IV

Tests for the identification of Xylella fastidiosa and its subspecies

A. Tests for the screening and identification of the presence of Xylella fastidiosa

• 1. Real time PCR based on Harper et al., 2010 (and erratum 2013) ([^E0007]);

• 1. Loop-mediated isothermal amplification (LAMP) based on primers developed by Harper et al. 2010 (and erratum 2013) ([^E0008]);

• 1. Real time PCR based on Ouyang et al., 2013 ([^E0009]);

• 1. Conventional PCR based on Minsavage et al. 1994 ([^E0010]).

B. Molecular tests for the identification of the subspecies of Xylella fastidiosa

• 1. Multi Locus Sequence Typing (MLST) based on Yuan et al., 2010 determining all subspecies ([^E0011]);

• 1. PCR based on Hernandez-Martinez et al., 2006 determining the subspecies fastidiosa, multiplex and sandyi ([^E0012]);

• 1. PCR based on Pooler & Hartung 1995 determining the subspecies pauca ([^E0013]);

• 1. Real time PCR based on Dupas et al. 2019, determining all subspecies ([^E0014]);

• 1. Real time PCR based on Hodgetts et al. 2021, determining all subspecies ([^E0015]).

ANNEX V

Templates for the reporting of the results of the surveys carried out pursuant to Articles 10 and 15 in demarcated areas

PART A

Template for the report of results of statistically based annual surveys

Instructions on how to fill in the template

Explain the assumptions for the survey design. Summarise and justify:

• — The target population, epidemiological unit and inspection units;

• — The detection method and method sensitivity;

• — The risk factor(s), indicating the risk levels and corresponding relative risks and proportions of host plant population.

• For column 1: Indicate the name of the geographical area, outbreak number or any information that allows the identification of the Demarcated Area (DA) concerned and the date when it was established.

• For columns 2 and 3: Indicate the size of the DA before the start of the survey and any relevant update.

• For column 4: Indicate the approach: Eradication (E), Containment (C). Please, include as many rows as necessary, depending on the number of DA and the approaches these areas are subject to.

• For column 5: Indicate the zone of the DA where the survey was carried out, including as many rows as necessary: Infected/infested zone (IZ) or buffer zone (BZ), using separate rows. When applicable, indicate the area of the IZ where the survey was carried out (e.g. last 5 km adjacent to the BZ, around nurseries, etc.) in different rows.

• For column 6: Indicate the survey sites, using more than one row if needed. Please, always report the surveys carried out in nurseries in a different row. When using the option “other”, please specify what it is:
• 1. Open air (production area): 1.1 field (arable, pasture); 1.2. orchard/vineyard; 1.3. nursery; 1.4. forest;
• 1. Open air (others): 2.1. private gardens; 2.2. public sites; 2.3. conservation area; 2.4. wild plants in areas other than conservation areas; 2.5. other, with specification of the particular case (e.g. garden centre, etc.);
• 1. Physically closed conditions: 3.1. greenhouse; 3.2. private site, other than greenhouse; 3.3. public site, other than greenhouse; 3.4. other, with specification of the particular case (e.g. garden centre).

• For column 7: Indicate the months of the year when the surveys were carried out.

• For column 8: Indicate the chosen target population providing accordingly the list of host species and area covered. The target population is defined as the ensemble of inspection units. Its size is typically defined for agricultural areas as hectares, but it could be lots, fields, greenhouses, etc. Please justify the choice made in the underlying assumptions in column 23 (“Comments”). Indicate the inspection units surveyed. “Inspection unit” means plants, plant parts, commodities, materials, pest vectors that had been scrutinised for identifying and detecting the pests.

• For column 9: Indicate the epidemiological units surveyed, indicating its description and unit of measurement. “Epidemiological unit” means a homogeneous area where the interactions between the pest, the host plants and the abiotic and biotic factors and conditions would result into the same epidemiology, should the pest be present. The epidemiological units are a subdivision of the target population which are homogenous in terms of epidemiology and which include at least one host plant. In some cases the whole host population in a region/area/country may be defined as epidemiological unit. They could be NUTS regions, urban areas, forests, rose gardens or farms, or hectares. The choice shall be justified in the underlying assumptions.

• For column 10 Indicate the methods used during the survey including the number of activities in each case. Indicate with N/A when the information of certain column is not available.

• For column 11: Indicate an estimation of the sampling effectiveness. Sampling effectiveness means the probability of selecting infected plant parts from an infected plant. For vectors, it is the effectiveness of the method to capture a positive vector when it is present in the survey area. For soil, it is the effectiveness of selecting a soil sample containing the pest when the pest is present in the survey area.

• For column 12: “Method sensitivity” means the probability of a method to correctly detect pest presence. The method sensitivity is defined as the probability that a truly positive host tests positive. It is the multiplication of the sampling effectiveness (i.e. probability of selecting infected plant parts from an infected plant) by the diagnostic sensitivity (characterised by the visual inspection and/or laboratory test used in the identification process).

• For column 13: Provide the risk factors in different rows, using as many rows as necessary. For each risk factor indicate the risk level and corresponding relative risk and proportion of host population.

• For column B: Indicate the details of the survey. Indicate with N/A when the information of certain column is not applicable. The information to be provided in these columns is related to the information included in the column 10 “Detection methods”.

• For column 19: Indicate the number of samples found positive, negative or undetermined. “Undetermined” are those analysed samples for which no result was obtained due to different factors (e.g. below detection level, unprocessed sample-not identified, old, etc.).

• For column 20: Indicate the outbreak notifications of the year when the survey took place. The outbreak notification number does not need to be included when the competent authority has decided that the finding is one of the cases referred to in Articles 14(2), 15(2) or 16 of Regulation (EU) 2016/2031. In this case, please indicate the reason for not providing this information in column 21 (“Comments”).

• For column 21: Indicate the sensitivity of the survey, as defined in ISPM 31. This value of the achieved confidence level of pest freedom is calculated based on the inspections (and/or samples) performed given the method sensitivity and the design prevalence.

• For column 22: Indicate the design prevalence based on a pre-survey estimate of the likely actual prevalence of the pest in the field. The design prevalence is set as a goal of the survey and corresponds to the compromise the risk managers are making between the risk of having the pest and the resources available for the survey.

PART B

Template for the report of results of surveys carried out for insect vectors of Xylella fastidiosa

Instructions on how to fill in the template

• For column1: Indicate the name of the area, outbreak number or any information that allows identifying the Demarcated Area (DA) and the date it was established.

• For column 2: Indicate: Eradication (E), Containment (C). Please include as many rows as necessary, depending on the number of DA and the approaches these areas are subject to.

• For column 3: Indicate the zone of the DA where the survey was carried out: Infected/infested zone (IZ) or buffer zone (BZ), using separate rows. When applicable, indicate the area of the IZ where the survey was carried out (e.g. last 5 km, around nurseries, etc.).

• For column 4: Indicate the list of vector species of the pest indicated in the first column, using different rows per vector.

• For column 5: Indicate only if applicable.

• For column 6: Indicate the type of vector capture method. When more than one method is used for the same vector, provide the data in separate rows.

• For column 7: Indicate the number of traps or other capture method, using a separate row per method.

• For column 8: Indicate when the traps or capture method were checked (e.g. once per week, once per month, four times per year, etc.).

• For column 9: Indicate the months of the year when the traps are checked.

• For column 10: Indicate the number of samples collected (a sample may contain several vectors).

• For column 11: Indicate the total number of vector trapped. Please include only the number of the vector of interest, no bycatch.

• For column 13: Indicate the number of vector samples analysed for the pest, applicable when a sample is composed by more than one vector.

• For column 16: The number of undetermined samples, namely samples analysed but where due to different factors no result was obtained (e.g. below detection level, etc.).’

[^E0001]: Guidelines for pest eradication programmes – Reference Standard ISPM No 9 by the Secretariat of the International Plant Protection Convention, Rome. Published 15 December 2011.

[^E0002]: The use of integrated measures in a systems approach for pest risk management – Reference Standard ISPM No 14 by the Secretariat of the International Plant Protection Convention, Rome. Published 8 January 2014.

[^E0003]: Commission Implementing Directive 2014/98/EU of 15 October 2014 implementing Council Directive 2008/90/EC as regards specific requirements for the genus and species of fruit plants referred to in Annex I thereto, specific requirements to be met by suppliers and detailed rules concerning official inspections, OJ L 298, 16.10.2014, p. 22.

[^E0004]: Council Directive 2008/90/EC of 29 September 2008 on the marketing of fruit plant propagating material and fruit plants intended for fruit production, OJ L 267, 8.10.2008, p. 8.

[^E0005]: Commission Implementing Decision (EU) 2017/925 of 29 May 2017 temporarily authorising certain Member States to certify pre-basic material of certain species of fruit plants, produced in the field under non-insect proof conditions, and repealing Implementing Decision (EU) 2017/167, OJ L 140, 31.5.2017, p. 7.

[^E0006]: ISPM No 4 ‘Requirements for the establishments of pest free areas’.

[^E0007]: DOI: 10.1094/PHYTO-06-10-0168.

[^E0008]: DOI: 10.1094/PHYTO-06-10-0168.

[^E0009]: DOI: 10.1371/journal.pone.0081647.

[^E0011]: DOI: 10.1094/PHYTO-100-6-0601.

[^E0012]: DOI: 10.1094/PD-90-1382.

[^E0013]: DOI: 10.1007/BF00294703.

[^E0014]: DOI: 10.3389/fpls.2019.01732.

[^E0015]: DOI: 10.1111/jam.14903.