LegalizeCommission Implementing Regulation (EU) 2024/771 of 29 February 2024 amending Regulation (EC) No 152/2009 laying down the methods of sampling and analysis for the official control of feed
This is the act as published. Later amendments are not folded into the text below: each one is a separate act in this repository and an entry in this law's history.
The principles described in the “Guidance Document on Measurement Uncertainty for Laboratories performing PCDD/F and PCB Analysis using Isotope Dilution Mass Spectrometry” (https://food.ec.europa.eu/system/files/2017-05/animal-feed-guidance_document_pcdd-f_pcb_en.pdf) shall be followed when applicable.
The concept of “upper-bound” requires using the limit of quantification for the contribution of each non-quantified congener. The concept of “lower-bound” requires using zero for the contribution of each non-quantified congener. The concept of “medium-bound” requires using half of the limit of quantification calculating the contribution of each non-quantified congener.
Duplicate analysis: Separate analysis of the analytes of interest using a second aliquot of the same homogenised sample. In general, the requirements for duplicate analysis as provided for in Annex II, Chapter C, point 3 apply. However, for methods with the use of 13C-labelled internal standard for the relevant analytes, the duplicate analysis is only necessary if the result of the first determination is not compliant. The duplicate analysis is necessary to exclude the possibility of internal cross-contamination or an accidental mix-up of samples. In case the analysis is performed in the course of a contamination incident, confirmation by duplicate analysis may be omitted in case the samples selected for analysis are through traceability linked to the contamination incident and the level found is significantly above the maximum level.
The concept of “upper-bound” requires using the limit of quantification for the contribution of each non-quantified congener to the Toxic Equivalent (TEQ). The concept of “lower-bound” requires using zero for the contribution of each non-quantified congener to the TEQ. The concept of “medium-bound” requires using half of the limit of quantification calculating the contribution of each non-quantified congener to the TEQ.
In general, the requirements for duplicate analysis as provided for in Annex II, Chapter C, point 3 apply. However, for confirmatory methods with the use of 13C-labelled internal standard for the relevant analytes, the duplicate analysis is only necessary if the result of the first determination is not compliant. The duplicate analysis is necessary to exclude the possibility of internal cross-contamination or an accidental mix-up of samples. In case the analysis is performed in the course of a contamination incident, confirmation by duplicate analysis may be omitted in case the samples selected for analysis are through traceability linked to the contamination incident and the level found is significantly above the maximum level.
Identical explanation and requirements for duplicate analysis for control of action thresholds as in footnote 5 for maximum levels.
Regulation (EC) No 183/2005 of the European Parliament and of the Council of 12 January 2005 laying down requirements for feed hygiene (OJ L 35, 8.2.2005, p. 1).
Bioanalytical methods are not specific to those congeners included in the TEF-scheme. Other structurally related AhR-active compounds may be present in the sample extract which contribute to the overall response. Therefore, bioanalytical results cannot be an estimate but rather an indication of the TEQ level in the sample.
“Guidance Document on Measurement Uncertainty for Laboratories performing PCDD/F and PCB Analysis using Isotope Dilution Mass Spectrometry” (https://food.ec.europa.eu/system/files/2017-05/animal-feed-guidance_document_pcdd-f_pcb_en.pdf), “Guidance Document on the Estimation of LOD and LOQ for Measurements in the Field of Contaminants in Feed and Food” (https://food.ec.europa.eu/system/files/2016-10/cs_contaminants_sampling_analysis-report_2004_en.pdf).
With respect to the maximum levels.
Current requirements are based on the TEFs published in: M. Van den Berg et al, Toxicol Sci 93 (2), 223–241 (2006).
Congeners often found to co-elute are for example PCB 28/31, PCB 52/69 and PCB 138/163/164. For GC-MS also possible interferences from fragments of higher chlorinated congeners shall be considered.
The principles as described in the “Guidance Document on the Estimation of LOD and LOQ for Measurements in the Field of Contaminants in Feed and Food” (https://data.europa.eu/doi/10.2787/8931) shall be followed when applicable.
It is highly recommendable to have a lower contribution of the reagent blank level to the level of a contaminant in a sample. It is in the responsibility of the laboratory to control the variation of blank levels, in particular, if the blank levels are subtracted.
See footnote 9.
Use of all six 13C-labelled analogues as internal standards required.
ANNEX VI
‘ANNEX VII
METHOD OF CALCULATING THE ENERGY VALUE OF POULTRY FEED
1. METHOD OF CALCULATION AND EXPRESSION OF ENERGY VALUE
The energy value of compound poultry feed must be calculated in accordance with the following formula set out on the basis of the percentages of certain analytical components of the feed. This value is to be expressed in megajoules (MJ) of metabolisable energy (ME), corrected for nitrogen, per kilogram of compound feed:
MJ/kg of ME = 0,1551 × % crude protein + 0,3431 × % crude fat + 0,1669 × % starch + 0,1301 × % total sugar (expressed as sucrose).
2. TOLERANCES APPLICABLE TO DECLARED VALUES
If the official inspection reveals a discrepancy (increased or reduced energy value of the feed) between the result of the inspection and the declared energy value, a tolerance of 0,4 MJ/kg of ME shall be permitted.
3. EXPRESSION OF RESULT
After application of the above formula, the result obtained must be given to one decimal place.
4. SAMPLING AND ANALYSIS METHODS
Sampling of the compound feed and determination of the content of analytical components indicated in the method of calculation must be performed in accordance with the Union sampling methods and analysis methods for the official control of feed respectively.
The following are to be applied:
- — for determining the crude fat content: procedure B of the method for the determination of crude oils and fats, laid down in Part G of Annex III,
- — for determining the starch content: the polarimetric method, laid down in Part K of Annex III
METHOD OF CALCULATING THE ENERGY VALUE IN FEED MATERIALS AND COMPOUND FEED FOR CATS AND DOGS
The energy value in feed materials and compound feed for cats and dogs is to be calculated in accordance with EN 16967 Animal feeding stuffs: Methods of sampling and analysis – Predictive equations for metabolisable energy in feed materials and compound feed (pet food) for cats and dogs including dietetic food.
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ELI: http://data.europa.eu/eli/reg_impl/2024/771/oj
ISSN 1977-0677 (electronic edition)
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