S.I. No. 297/1994 - European Communities (Trade in Bovine Breeding Animals, Their Semen, Ova and Embryos) Regulations, 1994

These provisions shall not apply to sero-positive bulls which, prior to their first vaccination in accordance with Council Directive 88/407/EEC, as amended at the insemination centre, gave a negative reaction to the serum neutralization test or the ELISA test for infectious bovine rhinotracheitis or infectious pustular vulvo-vaginitis;

Sero-positive bulls referred to in the second subparagraph of Article 4 (1) of Council Directive 88/407/EEC must be isolated, since their semen may be the subject of intra-Community trade in accordance with the provisions for trade in semen from such bulls.

SCHEDULE H CONDITIONS FOR THE APPROVAL OF AN OVA/EMBRYO COLLECTION AND OVA/EMBRYO PRODUCTION TEAM

Regulation 12.

In order to be granted approval each embryo collection team shall fulfill the following conditions:

( a ) The collection, processing and storage of embryos shall be carried out either by a team veterinarian or under his responsibility by one or more technicians who are competent and trained by the team veterinarian in methods and techniques of hygiene.

( b ) The embryo collection team shall be placed under the general supervision and authority of an official veterinarian.

( c ) The embryo collection team shall have at its disposal permanent or mobile laboratory facilities where embryos can be examined, processed and packed, consisting of at least a work surface, a microscope and cryogenic equipment.

( d ) In the case of a permanently sited laboratory, the embryo collection team shall have at its disposal—

• (i) a room where embryos can be manipulated which is adjacent to but physically separate from the area used to handle the donor animals during collection, and

• (ii) a room or area equipped for cleansing and sterilising instruments and equipment used in embryo collection and manipulation.

• (iii) Where micromanipulation of the embryo which involves penetration of the zona pellucida is to be carried out, this shall be done in suitable laminar-flow facilities which shall be properly cleaned and disinfected between batches.

( e ) The embryo collection team shall have at its disposal in the case of a mobile laboratory a specially equipped part of the vehicle consisting of two separate sections—

• (i) one for the examination and manipulation of embryos which shall be a clean section, and

• (ii) the other for accommodating equipment and materials used in contact with the donor animals.

A mobile laboratory shall always have contact with a permanently sited laboratory to ensure the sterilisation of its equipment and the provision of fluids and other products necessary for the collection and manipulation of embryos.

( f ) To be approved as a team for the production and processing of embryos derived by in vitro fertilization and/or in vitro culture, an embryo production team must fulfil the following additional requirements:

• (i) the personnel shall be trained in appropriate disease control and laboratory techniques, particularly in procedures for working in sterile conditions;

• (ii) it shall have at its disposal a permanently-sited processing laboratory which must:

—have adequate equipment and facilities, including a separate room for recovering oocytes from ovaries and separate rooms or areas for processing oocytes and embryos, and storing embryos and

—have laminar-flow facilities under which all oocytes, semen and embryos must be processed, however, the centrifugation of semen may be carried out outside the laminar-flow facility, as long as full hygienic precautions are taken;

• (iii) where oocytes and other tissues are to be collected in an abattoir, it must have at its disposal suitable equipment for the collection and transport of the ovaries and other tissues to the processing laboratory in a hygienic and safe manner.

( g ) It is the responsibility of the team veterinarian to ensure the welfare of the breeding female animals concerned. In particular embryos shall only be collected from breeding females to which suitable epidural anaesthesia have been administered which will have effect during the entire process of embryo collection.

SCHEDULE I CONDITIONS RELATING TO THE COLLECTION OR PRODUCTION, PROCESSING, STORAGE AND TRANSPORT OF OVA/EMBRYOS BY AN APPROVED EMBRYO COLLECTION OR PRODUCTION TEAM

Regulation 12.

( a ) Embryos shall be collected and processed by an approved collection team without coming into contact with any other consignment of embryos not meeting the requirements of these Regulations.

( b ) Embryos shall be collected in a place which is isolated from other parts of the premises or holding and which shall be in good repair and easy to cleanse and disinfect.

( c ) Embryos shall be processed (examined, washed, treated and placed in identified and sterile containers) in either a permanent laboratory facility or a mobile laboratory facility, which is not situated in a zone subject to prohibition or quarantine measures.

( d ) All implements which come into contact with the embryos or the donor animal during collection and processing shall be disposable or shall be properly disinfected or sterilised prior to use.

( e ) Products of animal origin used during collection of the embryos and in the transport medium shall be obtained from sources which present no animal health risk or are to be so treated prior to use so that such risk is prevented. All media and solutions shall be sterilised by approved methods according to the recommendations of the manual of the International Embryo Transfer Society (IETS). Antibiotics may be added to the media in accordance with the IETS manual.

( f ) Storage flasks and transport flasks shall be properly disinfected or sterilized before the commencement of each filling operation.

( g ) The cryogenic agent used shall not have been previously used for other products of animal origin.

( h ) Each embryo container and the containers in which they are stored and transported shall be clearly code-marked in such a way that the date of collection of the embryos and the breed and identification of the donor sire and donor dam, as well as the registration number of the team can be readily established. The characteristics and form of this marking shall be in accordance with that specified by the Minister.

( i ) Each embryo shall be washed at least 10 times in a special fluid for embryos which shall be changed each time and which, unless decided otherwise, shall contain trypsin, in accordance with internationally recognised procedures. Each wash shall be a 100-fold dilution of the previous wash and a sterile micropipette shall be used to transfer the embryo on each occasion.

( j ) After the last wash each embryo shall be subjected to microscopic examination at a magnification of at least x 50 over its entire surface to determine that the 'zona pellucida' is intact and is free from any adherent material. Any micromanipulation which involves penetration of the zoma pellucida shall be carried out in the facilities approved for the purpose, and after the last wash and examination. Such micromanipulation may only be carried out on an embryo having an intact zoma pellucida.

( k ) Each consignment of embryos that has successfully undergone the examination provided for in subparagraph (j) shall be placed in a sterile container marked in accordance with subparagraph (h) and which shall be sealed immediately.

( l ) Each embryo shall, where appropriate, be frozen as soon as possible and stored in a place which is under the control of the team veterinarian and which is subject to regular inspection by an official veterinarian.

( m ) All products in the processing, washing, transportation media must be obtained from sources which present no animal health risk or are so treated prior to use that such risk is prevented.

( n ) Each collection team shall submit to an approved laboratory specified by the Minister routine samples of flushing fluids, washing fluids, disintegrated embryos, non-fertilized ova etc., resulting from its activities for official examination for bacterial and viral contamination. The procedure for collecting of samples, conducting such examinations, together with the standards to be achieved shall be in accordance with that specified by the Minister. If the standards laid down are not achieved the Minister shall withdraw approval.

( o ) Each collection team shall keep a record of its activities in respect of embryo collection during the 12 months before and 12 months after storage including:

• (i) the breed, age and identification of the donor animals concerned,

• (ii) the place of collection, processing and storage of embryos collected by the team,

• (iii) the identification of the embryos together with details of their destination if known, and

• (iv) details of micromanipulation techniques which involve penetration of the zona pellucida or other techniques such as in vitro fertilization and/or or in vitro culture which have been performed on the embryos. In the case of embryos derived by an in vitro fertilization, the identification may be done on the basis of a batch, but must contain details of the date and place of collection of ovaries and/or oocytes. It must also be possible to identify the herd of origin of the donor animals.

( p ) The conditions laid down in paragraphs (a) to (o) shall apply as appropriate to the collection, processing, storage and transport of ovaries, oocytes and other tissues for use in in vitro fertilization and/or in vitro culture. Furthermore, the following additional conditions shall apply:

• (i) when ovaries and other tissues are to be collected at an abattoir, the abattoir should be officially approved and under the control of an official veterinarian whose responsibility it is to carry out ante and post mortem inspection of donors;

• (ii) materials and equipment coming into direct contact with ovaries and other tissues shall be sterilised before use and after sterilization, used exclusively for those purposes. Separate equipment shall be used to handle oocytes and embryos from different batches of donor animals;

• (iii) ovaries and other tissues shall not be allowed to enter the processing laboratory until completion of the post mortem inspection of the batch. If relevant disease is found in the batch of donors, or in any animals slaughtered in the abattoir on that day, all tissues from that batch must be traced and discarded;

• (iv) the washing and examination procedure laid down in subparagraphs (i) and ( j ) shall be carried out after the culture procedure has been completed;

• (v) any micromanipulation which involves penetration of the zona pellucida shall be carried out in accordance with the provisions of subparagraph (i), after the procedures laid down in subparagraph (s) have been completed;

• (vi) only embryos from the same batch of donors should be stored in the same ampoule/straw.

Each embryo collection or production team shall ensure that the embryos are stored at suitable temperatures in premises approved for the purpose by the Minister.

In order to be approved these premises shall:

• (i) comprise at least one lockable room intended exclusively for embryo storage;

• (ii) be easy to cleanse and disinfect;

• (iii) have permanent records of all incoming and outgoing movements of embryos and the final destination of the embryos in particular shall be specified in such records;

• (iv) be subject to inspection by the official veterinarian.

The competent authority may authorise the storage of semen that fulfils the requirements of Directive 88/407/EEC in the approved storage premises.

Embryos for trade shall be transported in hygienic conditions in sealed containers from the approved storage premises until their arrival at their destination.

The containers shall be marked in such a way that the number coincides with the number on the animal health certificate.

SCHEDULE J CONDITIONS APPLYING TO DONOR ANIMALS

Regulation 12.

( a ) they shall have spent at least the previous 6 months within Community territory or in the third country of collection;

( b ) they shall have been present in the herd of origin for at least 30 days prior to collection;

( c ) they shall come from herds which are:

—officially tuberculosis free

—officially brucellosis free or brucellosis free

—enzootic bovine leucosis free

in derogation from the third indent, they may come from a herd which is not leucosis free but for which certification has been obtained that there has not been any clinical case of enzootic bovine leucosis during the past three years;

( d ) during the previous year, they shall not have been present in a herd which has shown any clinical sign of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis.

( a ) shall be kept in a holding which is not subject to veterinary prohibition or quarantine measures,

( b ) shall show no clinical signs of disease.

SCHEDULE K ZOOTECHNICAL CERTIFICATE

Regulation 20.

For trade in pure-bred breeding animals of the bovine species

Issuing body: ............................................................ ............................................................ .................................

Name of herd book: ............................................................ ............................................................ .......................

Entry No. in herd book: ............................................................ ............................................................ ................

System of identification (tag, tattoo, brand, earmark, sketch card): ............................................................ ...

Identification: ............................................................ ............................................................ ................................

Name of animal: ............................................................ ............................................................ .............................

Date of birth: ................................ Breed:.................................... Sex:............................................................ ...

Name and address of breeder: ............................................................ ............................................................ .....

............................................................ ............................................................ ...........

Name and address of owner: ............................................................ ............................................................ .......

............................................................ ............................................................ ...........

	Father Herd Book No................	Grandfather Herd Book No....................	Grandmother Herd Book No................
Pedigree:	Pedigree:	Pedigree:	Pedigree:
	Mother Herd Book No................	Grandfather Herd Book No....................	Grandmother Herd Book No................

The results of performance tests and results with origin of the assessment of the genetic value, on the animal itself and its parents and grandparents.

Known hereditary defects in the donor animal or its ancestors shall be indicated. (State none if applicable).

_______
Done at............................................................ .	on ............................................................ ................
	............................................................ ......................
	(Signature)
	NAME IN CAPITAL LETTERS AND TITLE OF SIGNATORY
	............................................................ ....................

An alternative may be provided in documentation accompanying the pure-bred breeding animal. In this event the competent authorities of the exporting country shall certify that the particulars required under paragraph 1 above are indicated in those documents, by the following formula:

"The undersigned certifies that these documents contain the particulars mentioned in Article 1 of Commission Decision 86/404/EEC."

SCHEDULE L ZOOTECHNICAL CERTIFICATE

Regulation 20.

For trade in the semen of pure-bred breeding animals

Issuing body: ............................................................ ............................................................ .................................

Name of herd book: ............................................................ ............................................................ .......................

Entry No. in herd book: ............................................................ ............................................................ .................

System of identification (tag, tattoo, brand, earmark, sketch card):............................................................ .....

Name of animal: ............................................................ ............................................................ .............................

Date of birth: ............................................................ ......Breed:............................................................ .................

Name and address of breeder: ............................................................ ............................................................ .......

............................................................ ............................................................ .......

Name and address of owner: ............................................................ ............................................................ ........

............................................................ ............................................................ ......

	Father Herd Book No...................	Grandfather Herd Book No....................	Grandmother Herd Book No................
Pedigree:	Pedigree:	Pedigree:	Pedigree:
	Mother Herd Book No...................	Grandfather Herd Book No. ....................	Grandmother Herd Book No................

Blood group............................................................ ............................................................ ....................................

The updated results of performance tests and updated results with origin of the assessment of the genetic value, on the animal itself and its parents and grandparents.

Known hereditary defects in the donor animal or its ancestors shall be indicated. (State none if applicable).

__________
Done at............................................................ .........	on ............................................................ ....
	............................................................ .............................
	(Signature)
	NAME IN CAPITAL LETTERS AND TITLE OF SIGNATORY
	............................................................ .........

Semen identification system (colour, number): ............................................................ ....................................

Identification: ............................................................ ............................................................ ................................

A.

Number of doses	Date(s) of collection	Identification donor animal (Name/Herd Book No.)	Breed

B.

Origin of semen:

Address of semen collection centre(s): ............................................................ ......................................................

............................................................ ............................................................ ............................................................ ....

Destination of semen:

Name and address of consignee: ............................................................ ............................................................ ....

............................................................ ............................................................ ............................................................ ...

Done at............................................................ .....	on ............................................................ ...............
	............................................................ ...................
	(Signature)
	NAME IN CAPITAL LETTERS AND TITLE OF SIGNATORY
	............................................................ ....................

An alternative certificate may be provided in documentation accompanying the semen. In this event the competent authorities of the exporting country shall certify that the particulars required under Paragraph 1 are indicated in those documents, by the following formula:

"The undersigned certifies that these documents contain the particulars mentioned in Article 1 of Commission Decision 88/124/EEC."

SCHEDULE M ZOOTECHNICAL CERTIFICATE

For trade in embryos of pure-bred breeding animals

A. Particulars on donor bull:

Issuing body: ............................................................ ............................................................ .................................

Name of herd book: ............................................................ ............................................................ .......................

Entry No. in herd book: ............................................................ ............................................................ ................

System of identification (tag, tattoo, brand, earmark, sketch card): ............................................................ ..

............................................................ ............................................................ ..........................................................

Name of animal: ............................................................ ............................................................ ..........................

Date of birth:............................................................ .. Breed: ............................................................ ............

Name and address of breeder: ............................................................ ............................................................ .....

............................................................ ............................................................ ...........................................................

Name and address of owner: ............................................................ ............................................................ .......

............................................................ ............................................................ ..........................................................

	Father Herd Book No...................	Grandfather Herd Book No. ..............	Grandmother Herd Book No. ..................
Pedigree:
	Mother Herd Book No. ...................	Grandfather Herd Book No. ...............	Grandmother Herd Book No. ..................

Blood group .......................................................

The updated results of performance tests and updated results with origin of the assessment of the genetic value, on the donor bull itself and its parents and grandparents: ............................................................ ..

Known hereditary defects in the donor animal or its ancestors should be indicated. (State none if applicable). ............................................................ ............................................................ ........................................................

B. Particulars on donor cow:

Issuing body: ............................................................ ............................................................ .................................

Name of herd book: ............................................................ ............................................................ .......................

Entry No. in herd book: ............................................................ ............................................................ ................

System of identification (tag, tattoo, brand, earmark, sketch card): ............................................................ .

............................................................ ............................................................ ..........................................................

Identification ............................................................ ............................................................ ................................

Name of animal: ............................................................ ............................................................ ...........................

Date of birth: ............................................................ ....... Breed: ............................................................ ............

Name and address of breeder: ............................................................ ............................................................ ...

............................................................ ............................................................ .........................................................

Name and address of owner: ............................................................ ............................................................ ......

............................................................ ............................................................ .........................................................

	Father Herd Book No....................	Grandfather Herd Book No....................	Grandmother Herd Book No..................
Pedigree:
	Mother Herd Book No....................	Grandfather Herd Book No....................	Grandmother Herd Book No.................

Blood group ............................................................ .

The updated results of performance tests and updated results with origin of the assessment of the genetic value, on the donor cow itself and its parents and grandparents.

Known hereditary defects in the donor animal or its ancestors should be indicated. (State none if applicable) ............................................................ ............................................................ .....................................

_________
Done at............................................................ ......	on ............................................................ ..............
	............................................................ ..................
	(Signature)
	NAME IN CAPITAL LETTERS AND TITLE OF SIGNATORY
	............................................................ ...................

(Schedule M continued)

®

Embryo identification system (number, colour): ............................................................ .......................................

Identification: ............................................................ ............................................................ .................................

Number of embryos per straw: ............................................................ ............................................................ ....

Number of embryos	Date(s) of insemination	Date of Collection	Identification of donor cow and donor bull (Herd Book No.)	Breed

Origin of the embryo(s):

Address of embryo collection centre(s): ............................................................ ....................................................

............................................................ ............................................................ ............................................................ ...

Destination of embryo(s):

Name and address of consignee: ............................................................ ............................................................ ....

............................................................ ............................................................ ............................................................ ...

Done at ............................................................ ....	on ............................................................ ..................
	............................................................ .......................
	(Signature)
	NAME IN CAPITAL LETTERS AND TITLE OF SIGNATORY
	............................................................ ......................

An alternative certificate may be provided in documentation accompanying the embryos. In this event the competent authorities of the exporting country shall certify that the particulars required under Paragraph 1 are indicated in those documents, by the following formula:

"The undersigned certifies that these documents contain the particulars mentioned in Article 2 of Commission Decision 88/124/EEC."

SCHEDULE N CONDITIONS WHICH SEMEN COLLECTED AT APPROVED CENTRES MUST SATISFY FOR THE PURPOSES OF TRADE

Regulation 20

( a ) show no clinical signs of disease on the day the semen is collected;

( b ) in the case of semen to be imported—

• (i) have not been vaccinated against foot-and-mouth disease during the 12 months prior to collection or

• (ii) have been vaccinated against foot-and-mouth disease during the 12 months prior to collection, in which case 5% (with a minimum of five straws) of each collection shall be submitted to virus isolation test for foot-and-mouth disease with negative results;

( c ) have not been vaccinated against foot-and-mouth disease within 30 days immediately prior to collection;

( d ) have been kept at an approved semen collection centre for a continuous period of at least 30 days immediately prior to the collection of the semen in the case of collections of fresh semen;

( e ) are not allowed to serve naturally;

( f ) are kept in semen collection centres which have been free from foot-and-mouth disease for at least three months prior to collection of the semen and 30 days after collection, or, in the case of fresh semen, until the date of dispatch and are situated in the centre of an area of 10 kilometres' radius in which for at least 30 days there has been no case of foot-and-mouth disease;

( g ) have been kept in semen collection centres which, during the period commencing 30 days prior to collection and ending 30 days after collection of the semen or, in the case of fresh semen, until the date of dispatch, have been free from those bovine diseases which are compulsorily noticeable in accordance with Annex E to Council Directive 64/432/EEC.

not less than:	500 IU per ml streptomycin, 500 IU per ml penicillin, 150 Ug per ml lincomycin, 300 Ug per ml spectinomycin.

An alternative combination of antibiotics with an equivalent effect against campylobacters, leptospires and mycoplasmas may be used.

Immediately after their addition the diluted semen shall be kept at a temperature of at least 5°C for a period of not less than 45 minutes.

• (i) be stored in approved conditions for a minimum period of 30 days prior to dispatch. This requirement shall not apply to fresh semen.

• (ii) be transported in flasks which have been cleaned and disinfected or sterilised before use and which have been sealed prior to dispatch from the approved storage facilities.

SCHEDULE O ANIMAL HEALTH CERTIFICATION FOR TRADE IN SEMEN

Regulation 20.

	No:....................................Original

Country of Collection: ............................................................ ............................................................ ...................

Competent authority: ............................................................ ............................................................ ....................

Competent local authority: ............................................................ ............................................................ ...........

I. Identification of semen:

............................................................ ............................................................ .............................................

Number of Straws	Date(s) of Collection	Identification of donor animal Name/Herd Book No.	Breed	Date of Birth

II. Origin of semen:

Address of semen collection centre(s): ............................................................ ...........................................

............................................................ ............................................................ .....................................................

............................................................ ............................................................ .....................................................

Approval number of semen collection centre(s):............................................................ .....................................

............................................................ ............................................................ ............................................................ ...

III. Destination of semen:

The semen will be sent from: ............................................................ .....................................................	The semen will be sent from: ............................................................ .....................................................
	(place of loading)
	to: ............................................................ ........................................
	(country and place of destination)
	by: ............................................................ .......................................
	(means of transport)
Name and address of consignor:............................................................ ...................................................	Name and address of consignor:............................................................ ...................................................
............................................................ ............................................................ ........................................... ............................................................ ............................................................ ...........................................	............................................................ ............................................................ ........................................... ............................................................ ............................................................ ...........................................
Name and address of consignee:............................................................ ..................................................	Name and address of consignee:............................................................ ..................................................
............................................................ ............................................................ ........................................... ............................................................ ............................................................ ...........................................	............................................................ ............................................................ ........................................... ............................................................ ............................................................ ...........................................

IV. I, the undersigned official veterinarian, certify that:

^(1) Delete as necessary.

• (i) which gave negative result to a serum neutralization test or an ELISA test for infectious rhinotracheitis or infectious pustular vulvo-vaginitis carried out in accordance with Directive 88/407/EEC^(1); or

^(1) Delete as necessary.

• (ii) which gave a positive result to the tests referred to at (i) but which had already given a negative reaction to these tests prior to a first vaccination in accordance with the Directive 88/407/EEC at the semen collection centre ^(1); or

^(1) Delete as necessary.

• (iii) which gave a positive result to a serum neutralization test or an ELISA test for infectious bovine rhinotracheitis or infectious pustular vulvo-vaginitis and have not been vaccinated in accordance with Directive 88/407/EEC^(1); and in which case the semen comes from a collection which has been subjected, with a negative result, to an examination by inoculation or a virus isolation test^(1) as referred to in the third subparagraph or Article 4 (1) of Directive 88/407/EEC in laboratory . . . ^(2);

^(1) Delete as necessary.

^(2) Name of approved laboratory.

• (i) which have not been vaccinated against foot-and-mouth disease within 12 months prior to collection^(1); or

• (ii) which have been vaccinated against foot-and-mouth disease within 12 months prior to collection, in which case the semen comes from a collection in which 5 per cent of each collection intended for trade (with a minimum of 5 straws) has been subjected, with negative results, to a virus isolation test for foot and mouth disease in . . .laboratory^(2).

^(2) Name of approved laboratory.

^(3) May be deleted for fresh semen.

Done at............................................................ .....	on ............................................................ .............. ............................................................ ...................
	(Signature) (Name in block letters)

SCHEDULE P ANIMAL HEALTH CERTIFICATION FOR TRADE IN EMBRYOS

Regulation 20

1. Consignor (name and full address)	Animal Health Certificate No. Original
	2. Member State of collection
3. Consignee (name and full address)	4. Competent Authority
NOTES (a) A separate certificate shall be issued for each consignment of embryos (b) The original of this certificate shall accompany the consignment to the place of destination	5. Competent Local Authority
6 Place of loading
7. Name and address of embryo collection team
8. Means of transport
9. Place and Member State of destination
	10. Registration number of embryo collection team
11. Number and code-mark of embryos containers

(a) Number of embryos	(b) Date(s) of collection	(c) Breed

• (i) (d) Embryos derived by natural/in vitro fertilization and subjected to/not

subjected to penetration of the zona pellucida 13. I, the undersigned official veterinarian, certify that:

( a ) the embryos described above were collected, processed and stored under conditions which comply with the standards laid down in Directive No. 89/556/EEC and, where consigned to a Member State which does not vaccinate against foot-and-mouth disease, with the requirements set out in the second indent of Article 4(1) of that Directive;

( b ) the embryos described above were sent to the place of loading in sealed container under conditions which comply with the provision of Directive No. 89/556/EEC.

Done at ............................................................ ....	............................................................ .....................
	Signature ............................................................ .....
	Name and qualification (in block letters):
	............................................................ ....................

---

^(1)This need not be completed for embryos collected, processed and stored before 1 March 1994.

^(2)Delete as appropriate.

SCHEDULE Q APPROVAL FOR BREEDING

Regulation 13

PART I

Breeding Animals:

Breeding animals which comply with the following conditions are approved for breeding:

• (1) female breeding animals,

• (2) purebred male breeding animals entered in the main section of an approved herd book for natural service,

• (3) other male breeding animals for natural service which comply with the following:—

( a ) Control of Bulls for Breeding Act, 1985 ,

( b ) Control of Bulls for Breeding (Permits) Regulations, 1985 ( S.I. No. 333 of 1986 ), and

( c ) Control of Bulls for Breeding (Permits) (Amendment) Regulations, 1990 ( S.I. No. 166 of 1990 ).

PART II

Semen of Breeding Animals for use in artificial insemination/fertilisation:

• (1) Applications for approval for breeding of semen of breeding animals shall be accompanied by:—

( a ) a zootechnical certificate in accordance with Schedule L in the case of semen of pure bred breeding animals;

( b ) in the case of semen from other breeding animals the ancestry and zootechnical information similar to that referred to in Schedule L shall be provided; the origin of this information shall be stated and be acceptable to the Minister;

( c ) a declaration that the donor animal is or will be blood typed and that a blood typing certificate will be available from an approved laboratory on request:

( d ) a declaration of known genetic defects in the animal itself or its ancestry, and also

( e ) in the case of semen for testing and genetic evaluation purposes, the name of the holder of an approval in accordance with Regulation 9.

• (2) Semen of breeding animals shall comply with the following conditions:—

( a ) Semen for unrestricted use:

The genetic merit of the semen for required traits as specified by the Minister must have been established in accordance with Part II of Schedule C.

( b ) Semen for the testing and genetic evaluation of untested bulls:

Semen from untested breeding animals may only be used for the purpose of testing and genetic evaluation of such breeding animals. The number of inseminations and if necessary the time period for the use of such semen may be specified by the Minister.

( c ) Semen for special breeding purposes:

Notwithstanding paragraph (2) (b) semen from untested breeding animals may be approved for special breeding purposes for use in individual herds at the discretion of the Minister.

In such cases the Minister may attach conditions as he thinks fit to such approvals.

PART III

• (1) Ova and embryos of breeding animals which comply with the following are approved for breeding—

( a ) Ova and embryos of pure bred breeding animals

( b ) Embryos of other breeding animals the result of fertilisation by semen approved for unrestricted use under Part II (2) (a).

SCHEDULE R Exemption from accompanying Zootechnical and Veterinary Certification — required under Regulation 20 (b)

Regulation 22

Name and Address of approved semen collection centre	............................................................ ..................
	............................................................ ..................
	............................................................ ..................
	............................................................ ..................
Name and Address of Consignee	............................................................ ..................
	............................................................ ..................
	............................................................ ...................
	............................................................ ...................

Details of semen:

	Name of Bull	HB Number	Breed	A.I. Code	No. Straws
1.
2.
3.
4.
5.

—the following declaration signed by a designated employee of the centre

"The semen referred to above was stored at the above named centre and has been released for supply to the above named consignee

"

Signed: .....................................

on behalf of approved centre

The above certificate shall accompany delivery of semen to the consignee.

GIVEN under my Official Seal, this 20th day of September, 1994.

JOE WALSH,

Minister for Agriculture, Food

and Forestry.

EXPLANATORY NOTE.

These Regulations (a) transpose into national law the provisions of Council Directives 93/52/EEC and 93/60/EEC and Commission Decisions 94/113/EC and 94/515/EC which deal with veterinary conditions relating to trade in and assessment for breeding of bovine breeding animals, their semen, ova and embryos and (b) integrate those provisions of S.I. No. 259 of 1993 (the European Communities (Trade in Bovine Breeding Animals, their Semen, Ova and Embryos) Regulations). S.I. No. 259 of 1993 is thereby revoked and replaced by these Regulations.