The Animal By-Products (Wales) Regulations 2003

Made: 29th October 2003

Coming into force: 31st October 2003

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 1 — Introduction

Title, application and commencement

1

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Interpretation

2

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Approvals etc.

3

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 2 — Collection, transportation, storage, handling, processing and disposal of animal by-products

Category 1 material

4

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Category 2 material

5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Category 3 material

6

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Mixing mammalian and non-mammalian by-products

7

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Collection, transportation and storage

8

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 3 — Restrictions on access to animal by-products and their use

Access to animal by-products

9

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Restrictions on use

10

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Pasture land

11

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Intra-species recycling of fish

12

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 4 — Approved premises and competent authorities

The competent authority

13

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Approval of premises

14

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Biogas and composting plants

15

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Composting catering waste on the premises on which it originates

16

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Processing and intermediate plants own checks

17

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sampling at processing plants

18

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sampling at biogas and composting plants

19

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Samples sent to laboratories

20

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Laboratories

21

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 5 — Placing animal by-products and processed products on the market

Placing on the market of processed animal protein and other processed products that could be used as feed material

22

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Placing on the market of petfood, dogchews and technical products

23

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Placing on the market of compost or digestion residues for use on agricultural land

24

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 6 — Derogations

Competent authority for Chapter V of the Community Regulation

25

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Derogations regarding the use of animal by products

26

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Collection centres

27

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Burial of pet animals

28

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Burial in the event of a disease outbreak

29

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Burning and burial of bees and apiculture products

30

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 7 — Records

Records

31

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Records for consigning, transporting or receiving animal by-products

32

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Records for burying or burning of animal by-products

33

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Records for disposal or use on premises

34

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Delivery records to be kept by operators of approved biogas and composting plants

35

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Treatment records for biogas and composting plants

36

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Records for approved laboratories

37

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Records to be kept for consignments of compost or digestion residue

38

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART 8 — Administration and enforcement

Grants of approvals

39

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Suspension, amendment or revocation of approvals, authorisations and registrations

40

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Appeals

41

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Notice requiring the disposal of animal by-products or catering waste

42

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Cleansing and disinfection

43

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Compliance with notices

44

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Powers of entry

45

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Obstruction

46

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Penalties

47

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Enforcement

48

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Service of notices and other documents

49

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Transitional provisions

50

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Amendments and Revocations

51

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

SCHEDULE 1 — Additional requirements for biogas and composting plants

PART 1

Premises

1

• (1) There must be —

• (a) a reception area in which untreated animal by-products (including catering waste) are received,

• (b) an area in which vehicles and containers are cleansed and disinfected with adequate facilities for doing this, and

• (c) a clean area in which treated compost or digestion residue are stored.

• (2) The clean area must be, adequately separated from the reception area and the area in which vehicles and containers are cleansed and disinfected so as to prevent contamination of the treated material. Floors must be laid so that liquid cannot seep into the clean area from the other areas.

• (3) The reception area must be easy to clean and disinfect and must have an enclosed and lockable place or container to receive and store the untreated animal by-products.

2

The animal by-products must be unloaded in the reception area and either —

• (a) treated immediately, or

• (b) stored in the reception area and treated without undue delay.

3

The plant must be operated in such a way that —

• (a) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and

• (b) partially treated material is not contaminted with material which has not been treated to the same extent or liquids arising from it.

4

The operator shall identify, control and monitor suitable critical points in the operation of the plant to demonstrate that —

• (a) these Regulations and the Community Regulation are complied with;

• (b) treated material is not contaminated by untreated or partially treated material or liquids arising from it; and

• (c) partially treated material is not contaminated with material which has not been treated to the same extent or liquids arising from it.

5

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART II — Treatment systems and parameters for catering waste

Composting plants

Biogas plants

SCHEDULE 2 — Testing Methods

PART I — METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Time of testing

1

Tests must be begun on receipt of the sample or on the first working day which allows this method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator at between 2°C and 8°C until required. If the sample has been refrigerated it must be removed from the refrigerator and stored at room temperature for at least one hour before the test is started.

Samples

2

Tests must be carried out using two 10 gram portions of each sample submitted for testing. Each 10 gram sample must be placed aseptically in a sterile container containing 90 ml Clostridium perfringens diluent consisting of 0.1% peptone and 0.8% sodium chloride at a pH of 7 and mixed thoroughly until the sample is evenly suspended.

Inoculations

3

For each portion of the sample 1 ml of solution must be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of Shahidi - Ferguson agar (SF agar)[^f00025] at a temperature of 47°C±1°C must be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.

4

Once the agar has set, each agar plate must be overlaid with a further 10 ml SF agar at a temperature of 47°C±1°C. Once the overlay has set and with the plate lids uppermost the plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Samples with colonies of Clostridium perfringens

5

After incubation each set of duplicate plates must be examined for colonies characteristic of Clostridium perfringens (black). The sample provisionally fails if any colonies characteristic of Clostridium perfringens are present, in which case the following procedure must be followed to establish whether or not the colonies are Clostridium perfringens.

6

In the case of each plate, 10 characteristic colonies of Clostridium perfringens must be subcultured on to a further SF agar plate. If there are less than 10 colonies on the plate, all characteristic colonies must be subcultured on to the further plate. The plates must be incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

7

If the surface area of the plates is overgrown and it is not possible to select well isolated characteristic colonies, 10 suspect colonies must be subcultured on to duplicate SF agar plates and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

8

One characteristic colony from each plate must be subcultured on to SF agar and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Subcultured colonies

9

After incubation each plate must be examined for colonies characteristic of Clostridium perfringens. All colonies characteristic of Clostridium perfringens must be —

• (a) stab inoculated into motility nitrate medium[^f00026]; and

• (b) inoculated into either lactose gelatin medium[^f00027] or charcoal gelatin discs[^f00028];

and incubated anaerobically at 37°C±1°C for 20 hours±2 hours.

Examination of subcultures

Motility

10

The motility nitrate medium must be examined for the type of growth along the stab line. If there is evidence of diffuse growth out into the medium away from the stab line, the bacteria must be considered to be motile.

Reduction of nitrate to nitrite

11

After examination of the motility nitrate medium, 0.2 ml to 0.5 ml of nitrite detection reagent must be added to it. The formation of a red colour confirms that the bacteria have reduced nitrate to nitrite. Cultures that show a faint reaction (i.e. a pink colour) must be discounted. If no red colour is formed within 15 minutes, a small amount of zinc dust must be added and the plate allowed to stand for 15 minutes. If a red colour is formed after the addition of zinc dust no reduction of nitrate to nitrite has taken place.

Production of gas and acid from lactose and liquefaction of gelatin

12

The lactose gelatin medium must be examined for the presence of small gas bubbles in the medium.

13

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

14

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

15

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Control Tests

16

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

17

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

18

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

19

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

PART II — METHODS FOR THE ISOLATION OF SALMONELLA

A. BACTERIOLOGICAL METHOD

Day 1

Day 2

Day 3

Day 4

Day 5

B. ELECTRICAL CONDUCTANCE METHOD

1

Tests must be begun on receipt of the sample or on the first working day which allows the following method to be completed. If the test is not begun on the day of receipt the sample must be stored in a refrigerator until required. If the sample has been refrigerated it must be stored at room temperature for at least four hours before the test is started.

Day 1

2

Tests must be carried out in duplicate using two 25 gram portions of each sample submitted for testing. Each 25 gram sample must be placed aseptically in a sterile container containing 225 ml Buffered Peptone Water/Lysine/Glucose (BPW/L/G)[^f00035] and incubated at 37°C for 18 hours.

Day 2

3

The incubated BPW/L/G must be added to Selenite Cystine Trimethylamine-N-Oxide Dulcitol (SC/T/D)[^f00036] and Lysine Decarboxylase Glucose (LD/G)[^f00037] media in electrical conductance cells or wells. For cells or wells containing more than 5 ml medium 0.2 ml of the BPW/L/G must be added and for cells or wells containing 5 ml or less medium 0.1 ml of the BPW/L/G must be added. Cells or wells must be connected to appropriate electrical conductance measuring equipment set to monitor and record changes in electrical conductance at 6 minute intervals over a 24 hour period. The temperature of cells and wells must be kept at 37°C.

Day 3

Day 4

5