The Bovine Semen (Wales) Regulations 2008

• (c) not less than 150 μg lincomycin per ml final dilution, and

• (d) not less than 300 μg spectinomycin per ml final dilution.

• (2) An alternative combination of antibiotics which has an equivalent effect against campylobacters, leptospires and mycoplasmas may be used.

4

The centre veterinarian must ensure that, immediately after the addition of antibiotics, the semen is kept at a temperature of at least 5°C for a period of not less than 45 minutes.

1

• (1) Prior to the initial dispatch of semen from bovine animals identified as serologically positive for BVD/MD, the centre veterinarian must ensure that a semen sample from each bovine animal is subjected to a virus isolation or virus antigen ELISA test for the BVD/MD virus.

• (2) In the event of a positive result, the centre veterinarian must ensure that the bovine animal is removed from the collection centre and its semen destroyed.

2

The centre veterinarian must ensure that frozen semen is kept in the semen quarantine facilities for at least 30 days before it leaves the centre.

3

The centre veterinarian must not allow any semen to leave the centre unless the premises where it was collected remain clear of—

• (a) foot and mouth disease; and

• (b) the bovine diseases listed in Annex E(I) to Directive 64/432/EEC

for 30 days after collection or, in the case of fresh semen, until the date of dispatch of the semen.

4

With regard to a bovine animal that—

• (a) did not belong to a herd officially free of enzootic bovine leukosis in accordance with Directive 64/432/EEC; or

• (b) was produced by a dam which did not, after removal of the bovine animal from it, test negative to a test carried out in accordance with Annex D (Chapter II) to Directive 64/432/EEC,

the centre veterinarian must not allow its semen to leave the centre until the bovine animal has reached the age of two years and has tested negative for enzootic bovine leukosis, under a serological test carried out in accordance with the procedure described in Annex D (Chapter II) to Directive 64/432/EEC.

1

• (1) The centre veterinarian must make a record of a bovine animal admitted that—

• (a) did not belong to a herd officially free of enzootic bovine leukosis in accordance with Directive 64/432/EEC; or

• (b) was produced by a dam which did not, after removal of the bovine animal from it, test negative to a test carried out in accordance with Annex D (Chapter II) to Directive 64/432/EEC.

• (2) The centre veterinarian must pass a copy of any record made under sub-paragraph (1) to the centre veterinarian of any collection centre to which the bovine animals or their semen may move, not later than the date of such move.

2

The centre veterinarian must ensure that each individual collection of semen collected at the centre is clearly marked in such a way that the following information can be readily established—

• (a) the date the semen was collected;

• (b) the identity of the donor bovine animal under the cattle identification regulations;

• (c) the breed of the donor bovine animal; and

• (d) the licence number of the centre.

1

The centre veterinarian must ensure that all bovine animals kept at a domestic collection centre must be subjected at least once a year to the following tests—

• (a) for bovine tuberculosis, an intradermal tuberculin test, carried out in accordance with the procedure laid down in Annex B to Directive 64/432/EEC;

• (b) for bovine brucellosis, a serological test carried out in accordance with the procedure described in Annex C to Directive 64/432/EEC; and

• (c) for enzootic bovine leukosis, a serological test carried out in accordance with the procedure described in Annex D (Chapter II) to Directive 64/432/EEC.

2

• (1) If any of the above tests is positive, the centre veterinarian must ensure that the bovine animal is isolated and the semen collected from it since the last negative test is destroyed.

• (2) The centre veterinarian must ensure that—

• (a) semen collected from all other bovine animals at the centre since the date when the positive test was carried out is held in separate storage; and

• (b) such semen is not used or supplied until the health status of the centre has been restored to the level required by the Directive and these Regulations.

1

The centre veterinarian must ensure that—

• (a) semen processed at the centre is semen collected—

• (i) at a licensed collection centre;

• (ii) at a collection centre approved under the Directive in another part of the United Kingdom or in a member State;

• (iii) at a domestic collection centre;

• (iv) at unlicensed premises in accordance with these Regulations; or

• (v) lawfully in another part of the United Kingdom;

• (b) semen is not stored with semen of a different health status and semen must be identifiable by a distinct marking that is different to marking used at licensed collection and storage centres;

• (c) products of animal origin used in the processing of semen, including additives or diluents, are obtained from sources which present no animal health risk or are so treated prior to use that such risk is removed;

• (d) the cryogenic agent used has not been previously used for other products of animal origin;

• (e) each individual dose of semen is sealed, numbered and clearly marked so that the following information can be readily established—

• (i) the date the semen was collected;

• (ii) the identity of the donor bovine animal under the cattle identification regulations;

• (iii) the breed of the donor bovine animal; and

• (iv) the licence number of the centre where the semen was collected (if applicable).

2

The centre veterinarian may not admit semen not collected at the centre for processing unless it is accompanied by—

• (a) the documents specified in regulation 14(4); and

• (b) if the semen comes from unlicensed premises, the documents specified in Schedule 7, paragraph 1(c).

3

• (1) Subject to sub-paragraph (2), the centre veterinarian must ensure that the antibiotics streptomycin, penicillin, lincomycin and spectinomycin are added to produce the following concentrations in the final diluted semen—

• (a) not less than 500 μg streptomycin per ml final dilution,

• (b) not less than 500 International Units penicillin per ml final dilution,

• (c) not less than 150 μg lincomycin per ml final dilution, and

• (d) not less than 300 μg spectinomycin per ml final dilution.

• (2) An alternative combination of antibiotics with an equivalent effect against campylobacters, leptospires and mycoplasms may be used.

4

The centre veterinarian must ensure that, immediately after the addition of antibiotics, the semen is kept at a temperature of at least 5°C for a period of not less than 45 minutes.

1

The centre veterinarian must ensure that frozen semen is kept in the semen quarantine unit for at least 30 days before it leaves the centre.

2

The centre veterinarian must not allow any semen to leave the domestic collection centre unless the premises where it was collected remain clear of—

• (a) foot and mouth disease; and

• (b) the bovine diseases listed in Annex E(I) to Directive 64/432/EEC

for 30 days after collection or, in the case of fresh semen, until the date of dispatch of the semen.

3

With regard to a bovine animal that—

• (a) did not belong to a herd officially free of enzootic bovine leukosis in accordance with Directive 64/432/EEC; or

• (b) was produced by a dam which did not, after removal of the bovine animal from it, test negative to a test carried out in accordance with Annex D (Chapter II) to Directive 64/432/EEC,

the centre veterinarian must not allow its semen to leave the centre until the bovine animal has reached the age of two years and has tested negative for enzootic bovine leukosis, under a serological test carried out in accordance with the procedure described in Annex D (Chapter II) to Directive 64/432/EEC.

4

With regard to a bovine animal vaccinated against foot-and-mouth more than 30 days before, but within 12 months of, a collection, the centre veterinarian must not allow its semen to leave the centre unless 5% (with a minimum of five straws) of each collection have tested negative to a virus isolation test for foot-and-mouth disease.

The bovine animals have—

• (a) completed 28 days' quarantine in—

• (i) a licensed quarantine centre; or

• (ii) a quarantine centre approved in accordance with paragraph 1(a) of Chapter 1 of Annex B to the Directive by the competent authority of a member State or part of the United Kingdom,

where only other cloven-hoofed animals having at least the same health status were present; or

• (b) undergone the tests referred to in Part 2 of Schedule 3 during the previous 12 months and have been kept in—

• (i) another licensed collection centre, or

• (ii) a semen collection centre authorised under the Directive in accordance with paragraph 5 of Chapter 1 of Annex B to the Directive, in the case of movement of a bovine animal kept in a semen collection centre authorised under the Directive in a member State or part of the United Kingdom.

Editorial notes

Entry of bovine animals to licensed quarantine centres, licensed collection centres, domestic collection centres, or to unlicensed premises

Trade in semen to a member State